TABLE 1.

Strains, plasmids, and primers

Strain, plasmid, or primer	Relevant information	Source or reference
Bacterial strains
E. coli strains
DH5α	F− φ80lacZ M15 endA recA hsdR(rk− mk−) supE thi gyrA relA Δ(lacZYA-argF)U169	Laboratory stock
BL21(DE3)	F−ompT hsdSB(rB− mB−) gal dcm	Novagen
JM110	dam dcm mutant	Laboratory stock
BW25113	K-12 derivative; ΔaraBAD ΔrhaBAD	12
S. coelicolor
A3(2)M145	SCP1− SCB2− Pgl+	KCTCa
BG711	absC deletion mutant	This paper
BG712	A3(2) M145 carrying pYH713	This paper
BG713	absC deletion mutant carrying pYH713
BG714	absC deletion mutant carrying pYH714
Plasmids
pET24ma	p15A replication origin, T7 lac promoter, C-terminal His-tag coding, Kanr	40
pIBR25	pWHM3 carrying ermE* promoter (EcoRI/KpnI) from Saccharopolyspora erythraea	37
pYH712	pET24ma carrying PCR product of absC from S. coelicolor
pYH713	pIBR25 carrying PCR product of absC (SCO5405) from S. coelicolor
pYH714	pWHM3 carrying PCR product of SCO5405 and SCO5406 together with 200 bp upstream of SCO5406 as the promoter
pIJ773	aac(3)IV(Aprar) + oriT	12
Primers
5405SCf	CGTGGATCCATGGAGACCGAGACGGCCAC
5405SCb	ACGAAGCTTTCAACCGTTGCGGCGCAGCG
5406_200bp	ATATGGATCCGTCTTCGAGGTGCCCATCACC/ATATAAGCTTGATCAGGGACTCAGGGTCGTCb
5405Ecolif	CGTGGATCCATGGAGACCGAGACGGCCAC
5405Ecolib	ACGAAGCTTTCAGTGGTGGTGGTGGTGGTGACCGTTGCGGCGCAGCG
Del5405f	TAGTTCGCCTTTCAACCTTTTAGGTAGACTTCATTCATGATTCCGGGGATCCGTCGACC
Del5405b	CCGGGCCGGAACGGCCCGGGACATGTGATCAGGGACTCATGTAGGCTGGAGCTGCTTC
5405confirm	CGGTAATCTGCGGAGTGTGAC/GCACGCTCACGGTGCACGACGb
Del-scbRf	ATCCTTCCCGGGGGAGACATGAACAAGGAGGCAGGCATGATTCCGGGGATCCGTCGACC
Del-scbRb	ACGGCGGGTCGGTATCCGGTGCGGGCGCTTCGGCGGTCATGTAGGCTGGAGCTGCTTC
EMSA-citA	ACGGTGTCGGCCGGGAGGCGG/GTCTTCCCTCACCGACGTAGTb	300 bp (−300∼−1)
EMSA-sacA	CCCCTTCCGTGTCGCGCCGGG/GACAGTCTCCTTCATTTATGTb	300 bp (−300∼−1)
EMSA-aceE1	CCCTGGAGAAGGGCTGCGGAC/GTCGCCGCCTTCCTCAGTCGAb	300 bp (−300∼−1)
EMSA-aceE2	GGTGACGATCCCGGCTGTGTC/GCTGTTCCTTCGCTGTCAGAGb	300 bp (−300∼−1)

^aKCTC, Korean Collection for Type Culture.

^bForward/reverse.