TABLE 2.

Differentially expressed spots of absC mutant (BG711) in two-dimensional gel^a

Protein function	Regulator	Characteristic	Spot ID no.	Fold changeb	No. of matched peptides
Downregulated
Purine metabolism	SCO4928	Adenylate cyclase, CyaA	474	O	2
	SCO4068	Phosphoribosylamine-glycine ligase, PurD	407	O	10
	SCO4087	Phosphoribosylaminoimidazole synthetase, PurM	529	O	16
Pyrimidine metabolism	SCO4041	Uracil phosphoribosyltransferase, UraP	675	O	2
Pentose phosphate pathway	SCO2627	Ribose-5-phosphate isomerase B	822	O	10
Fatty acid metabolism/biosynthesis	SCO2387	ACP S-malonyltransferase, FabD	594	5.5	11
	SCO3079	Putative thiolase	442	O	9
	SCO5144	Acyl-CoA isomerase	654	7.7	2
Vitamin B6 metabolism	SCO1523	Pyridoxine biosynthesis protein, YaaD	586	O	2
	SCO4293	Threonine synthase	413	O	17
	SCO4366	Phosphoserine aminotransferase	453	O	8
Folate metabolism	SCO3401	Hydroxymethyldihydropteridine pyrophosphokinase, FolK	474	O	11
	SCO4824	Methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase, FolD	570	O	2
Glycolysis and TCA	SCO1945	Triosephosphate isomerase, TpiA	632	2.8	19
	SCO1947	Glyceraldehyde-3-phosphate dehydrogenase, Gap1	453, 474, 483	O	8, 5, 4
	SCO2180	Dihydrolipoamide dehydrogenase, PdhL	389	O	38
	SCO2181	Dihydrolipoamide succinyltransferase- SucB	663	11	9
	SCO4808	Succinyl-CoA synthetase subunit beta, SucC	492	4.9	2
	SCO4827	Malate dehydrogenase, Mdh	545	O	20
	SCO7511	Glyceraldehyde 3-phosphate dehydrogenase, Gap2	511	O	6
Antioxidant mechanism	SCO2633	Superoxide dismutase [Fe-Zn], SodF	689	2.4	6
	SCO4164	Thiosulfate sulfurtransferase, CysA	578	7.5	13
	SCO4394	Iron repressor, DesR	633	O	2
	SCO5419	Thioredoxin, TrxA4	511	O	12
Type I polyketide biosynthesis	SCO6282	3-Oxoacyl-[acyl-carrier protein] reductase	653	2.8	11
CDA synthesis	SCO3231	CDA peptide synthetase II, CdaPS2	569	O	2
	SCO3247	Acyl-CoA oxidase	693	3	2
Upregulated
Glycolysis and TCA	SCO1946	Phosphoglycerate kinase, Pgk	317	5.3	5
	SCO1947	Glyceraldehyde-3-phosphate dehydrogenase, Gap1	360, 381	O	4, 4
	SCO2180	Dihydrolipoamide dehydrogenase, PdhL	282	O	19
	SCO4209	Phosphoglyceromutase, Pgm	573	O	11
	SCO5107	Succinate dehydrogenase, SdhA	191	25.1	2
	SCO5424	Acetate kinase, AckA	314	O	1
	SCO5425	Phosphate acetyltransferase, Pta	228	3.3	3
	SCO7000	Isocitrate dehydrogenase, Idh	177	O	27
	SCO7412	Pyruvate decarboxylase	556	4.4	1
Secondary metabolite signaling	SCO5113	ABC transport system lipoprotein, BldKB	189	6.2	19
	SCO5737	Guanosine pentaphosphate synthetase/polyribonucleotide nucleotidyltransferase, GpsI	173	O	11
Type I polyketide biosynthesis	SCO6275	Type I polyketide synthase	320	O	2
Amino acid metabolism	SCO1501	Alanine tRNA synthetase, AlaS	556	4.4	1
	SCO1595	Phenylalanyl-tRNA synthetase alpha subunit, PheS	317	5.3	2
	SCO2198	Glutamine synthetase I, GlnA	253	2.7	4
	SCO2210	Glutamine synthetase, GlnII	556	4.4	1
	SCO2241	Probable glutamine synthetase	253	2.7	7
	SCO5699	Prolyl tRNA synthetase, ProS	189	6.2	2
Pentose phosphate pathway	SCO1935	Transketolase A, TktA1	181	4	5
Antioxidant mechanism	SCO0379	Catalase, KatA	257	O	14
Other	SCO3820	Serine/threonine protein kinase	224	O	3

^aSpot ID, fold change, and number of matched peptides determined by mass spectrometric analysis are indicated for each classified protein.

^bSome protein spots appeared only in M145 or BG711 because they are under the detection limit of image analysis spot recognition criteria. These are indicated as “O” in fold change column.