TABLE 1.
E. coli strains and plasmids used in this work
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| CC118 λ Pm::lacZ | Rifr; CC118 with chromosomal mini-Tn5::Pm::lacZ insertion | 29 |
| BL21(DE3) | Carries T7 RNA polymerase under control of lacUV5 promoter | Novagen |
| DH5α | supE44 lacU169(φ80lacZΔM15) hsdR17 (rK− mK−) recA1 endA1 gyrA96 thi-1 relA1 | 23 |
| Plasmids | ||
| pJLR100 | Apr; Pm cloned in pEMBL9 | 46 |
| pGP1-2 | Kmr; contains an inducible T7 RNA polymerase genea | 58 |
| pERD103 | xylS IncQ Kmr | 45 |
| pET16b | Apr; protein expression vector | Novagen |
| pET-XylS-C | Apr; pET16b derivative used to produce His-tagged XylS C-terminal domain | 12 |
| pET-XylS | Apr; pET16b derivative used to produce His-tagged XylS | 12 |
| pET-XylS-N | Apr; pET16b derivative used to produce His-tagged XylS N-terminal domain | 12 |
| pBend2::Pm80 | Apr; pBend2 derivative carrying 86-bp DNA fragment containing Pm promoter | 19 |
a
pGP1-2 contains gene I (RNA polymerase) of phage T7 under the control of the inducible PL promoter of phage λ; pGP1-2 also contains the Plac promoter and the gene for the heat-sensitive λ repressor, cI857.