FIG. 4.

RNA primer syntheses by TtDnaG on different templates. The lanes with RNA primers synthesized are indicated by closed arrowheads (more significant) or open arrowheads (less significant), and the main RNA products are indicated with arrows. (A) RNA primers synthesized by TtDnaG on 16 templates with the sequence 5′-A₆XA₆-3′ (3′-OH terminus blocked) (lanes 1 to 16), with the exact sequences of X shown at the top of the figure, containing all 64 potential trinucleotides in total. (B) RNA primer syntheses by TtDnaG on templates with the sequence 5′-AATA₅XA₆-3′ (lanes 1 to 8), with X representing specific sequences derived from 5′-GGCCCA-3′, as shown at the top of the figure. (C) RNA primer syntheses by TtDnaG on 16 templates with the sequence 5′-AATA₅XA₆-3′ (lanes 1 to 16), with the exact sequences of X shown at the top of the figure, containing all 16 tetranucleotides composed entirely of cytosines and/or guanines. M, ssDNA markers as in Fig. 2.