TABLE 1.
Important templates used in this study
| Template | Sequence (5′ to 3′)a | Concentration |
|---|---|---|
| GA59 | GTTTTTTTAACTGGATAGCGGCAAAAAATTTTTATTTTATCCAGGTAATTGTGACTTTA | 1 μM |
| TC59 | TAAAGTCACAATTACCTGGATAAAATAAAAATTTTTTGCCGCTATCCAGTTAAAAAAAC | 1 μM |
| TC10 | ATTTTTTGCCGCTATCCAGT | 1 μM |
| TC10-9 | ATTTTTTGCCGC | 100 pM (SPR assay) |
| TC-M1 | ATTTTTTGTCGC | 100 pM (SPR assay) |
| TC-M2 | ATTTTTTGCCTC | 100 pM (SPR assay) |
a
The specific initiation sites for TtDnaG on the templates are underlined, while the possible nonspecific initiation sites are shown in italics. The mutated nucleotides in the initiation sites for TC-M1 and TC-M2 are shown in bold.