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Expression of pup91 abrogates Ino1 degradation. Ectopic expression of his6-pup91 abrogates degradation of endogenous Ino1. Equivalent cell numbers were harvested, lysed by bead beating, and clarified by microcentrifugation for 10 min at 16,000 × g. Antibodies to Ino1 are described elsewhere (9). DlaT is the loading control.
