TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| Strains | ||
| MG1655 | F− λ−ilvG rfb-50 rph-1 | 7 |
| MG1655Δ2 | MG1655 Δ(mqsR ygiT) | This study |
| MG1655Δ3 | MG1655 Δ(ygiV mqsR ygiT) | This study |
| BW23473 | Δ(lacIZYA argF)U169 rph-1 rpoS396(Am) robA1 creC510 hsdR514 ΔendA9 uidA(ΔMluI)::pir(wt) recA1 | 21 |
| BW25113 | lacIqrrnB3 lacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1 | 21 |
| BW25113 ΔmotA | BW25113 ΔmotA::kan | 4 |
| BW25113 ΔfliC | BW25113 ΔfliC::kan | 4 |
| BW25113 ΔmotA | BW25113 ΔmotA::kan | 4 |
| BW25113 ΔmqsR | BW25113 ΔmqsR::kan | 4 |
| BW25113 ΔmqsR Kans | BW25113 ΔmqsR | This study |
| BW25113 ΔmqsR-ygiT | BW25113 Δ(mqsR ygiT) | This study |
| BW25113 ΔmazF | BW25113 ΔmazF::kan | 4 |
| BW25113 ΔmazEF | BW25113 ΔmazEF::kan | This study |
| BW25113 ΔrelE | BW25113 ΔrelE::kan | 4 |
| BW25113 ΔrelBE | BW25113 ΔrelBE | H. Luidalepp |
| HM21 | F+dapA zde-264::Tn10 tet | 38 |
| HM21Δ | HM21 Δ(mqsR ygiT)::kan | This study |
| HM21Δ2 | HM21 Δ(mqsR ygiT) ΔlacZ::kan | This study |
| HM21Δ2 PTXlacZ | ΗΜ21 Δ(mqsR ygiT) ΔlacZ attλ::pPTX3 | This study |
| HM22 | F+dapA zde-264::Tn10 hipA7 cs | 38 |
| Plasmids | ||
| pKD13 | bla FRT aph FRT PS1 PS2 oriR6K | 14 |
| pKD46 | bla PBAD-gam bet exo pSC101 oriTS | 14 |
| pCP20 | bla cat cI857 λPR-flp pSC101 oriTS | 14 |
| pBAD33 | cat araC PBAD pACYC184 ori | 20 |
| pBRlacItac | bla lacIq Ptac pBR322 ori | 40 |
| pAH125 | kan lacZ attλ oriR6K | 21 |
| pINT-ts | bla intλ cI857 λPR-flp pSC101 oriTS | 21 |
| pGEM9Zf | bla T7P SP6P orif1, pGEM ori | Promega |
| pTX3 | pBAD33-mqsR | This study |
| pAT3 | pBRlacItac-ygiT | This study |
| pPTX3 | pAH125-PmqsRygiT | This study |
| pTX4 | pGEM9Zf-mqsR | This study |
| pTX5 | pGEM9Zf-mqsRygiT | This study |