FIG. 2.
BacA increases the sensitivity of S. meliloti Rm1021, R. leguminosarum 3841, and R. etli CE3 toward Bac71-35. (A and C) Strains of either the wild type (filled bars) or bacA mutants (open bars) were treated with defined concentrations of either Bac71-35 (A) or Bac71-16 (C) for 1 h at 30°C. (B) Wild-type strain with control plasmid (pRK404) or bacA mutant with pJG51A (S. meliloti bacA gene in pRK404) (filled bars) or bacA mutant with control plasmid (open bar) were treated as described for panel A. The CFU were determined after 48 (R. leguminosarum and R. etli), 72 (S. meliloti on LB), or 96 h (S. meliloti on GMS). All datasets shown are representative of trends observed in two independent experiments, and in each case the error bars represent the standard deviations from the means (n = 3) for one experiment.