TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or characteristic | Source or reference |
|---|---|---|
| R. leguminosarum bv. viciae | ||
| 3841 | Derivative of R. leguminosarum bv. viciae strain 300; Smr | 19 |
| LMB132 | 3841 with ΩTet cassette inserted into unique EcoRI site of bacA; Tcr | This work |
| RU4040 | 3841 bacA::pK19; Kmr | 22 |
| A34 | R. leguminosarum bv. viciae formerly known as 8401/pRL1JI; Smr | 7 |
| RU4043 | A34 bacA::pK19; Kmr | This work |
| R. leguminosarum bv. phaseoli | ||
| 4292 | Derivative of field bean isolate 8002 with sym plasmid pRP2J1; Rifr | 24 |
| RU4109 | 4292 bacA::pK19; Kmr | This work |
| R. etli | ||
| CE3 | Derivative of R. etli CFN42; Smr | 33 |
| LMB128 | CE3 with ΩSpec cassette inserted into unique StuI site of bacA; Spr | This work |
| S. meliloti | ||
| Rm1021 | Derivative of SU47; Smr | 30 |
| SmGF1 | Rm1021, bacA654::Spc (bacA null); Spr | 12 |
| E. coli | ||
| Fusion-Blue | endA1 hsdR17(rk12− mk12+) supE44 thi-1 gyrA96 relA1 lacF′[proA+B+lacIqZΔM15::Tn10(tet)] | Clontech Laboratories |
| DH5α | F−φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1 | Invitrogen |
| pHP45 | Plasmid containing ΩSpec or ΩTet cassette; Spr or Tcr | 8 |
| pJET1.2/blunt | PCR product cloning vector; Apr | Fermentas |
| pJQ200SK | pACYC derivative, P15A origin of replication; Gmr | 39 |
| pK19mob | pK19mob pUC19 derivative lacZ mob; Kmr | 41 |
| pLMB42 | pJET1.2/blunt containing R. etli CE3 bacA; Apr | This work |
| pLMB43 | pJET1.2/blunt containing R. leguminosarum bv. viciae 3841 bacA; Apr | This work |
| pLMB44 | R. etli CE3 bacA::ΩSp cloned in pJET2.1/blunt; Apr Spr | This work |
| pLMB45 | R. leguminosarum bv. viciae 3841 bacA::ΩTc cloned in pJET2.1/blunt; Apr Tcr | This work |
| pLMB46 | XhoI/XbaI fragment of pLMB44 cloned in pJQ200SK; Gmr Spr | This work |
| pLMB48 | XhoI/XbaI fragment of pLMB45 cloned in pJQ200SK; Gmr Tcr | This work |
| pRK2013 | ColEI replicon with RK2 tra genes, helper plasmid used for mobilizing plasmids; Kmr | 13 |
| pRU2048 | pK19mob containing internal fragment of R. leguminosarum bv. viciae 3841 bacA; Kmr | This work |
| pRU2049 | pK19mob containing internal fragment of R. leguminosarum bv. phaseoli 4292 bacA; Kmr | This work |
| pRK404 | Broad-host-range control plasmid; Tcr | 6 |
| pJG51A | pRK404 with S. meliloti Rm1021 bacA; Tcr | 15 |