TABLE 2.
Primers used in this study
| Primer | Sequence (5′ to 3′) | Function |
|---|---|---|
| BDbacAfor | TGATTACGCCAAGCTCAGCAGCCGATCGACCTTTCG | bacA PCR primer for internal gene fragment |
| BDbacArev | GCAGGCATGCAAGCTTTGCTGACCTGTCCGAAGGC | bacA PCR primer for internal gene fragment |
| p1060 | TTTACTAGTCGACACTCCCGAGACTCACAGT | Mapping primer for pK19 bacA and bacA |
| p1310 | GCGATGACCTTCTCGGCATCA | RL4439 (mdh) qRT-PCR primer |
| p1311 | CATGGCGTCGAGCGGATTG | RL4439 (mdh) qRT-PCR primer |
| p1346 | AAAGGGCGGCATTGGCAAG | pRL100162 (nifH) qRT-PCR primer |
| p1347 | AAGATCAGGCGCGTAGAATCGG | pRL100162 (nifH) qRT-PCR primer |
| p1348 | CAACGGAAACGATGGTGATCGC | pRL100205 (fixN1) qRT-PCR primer |
| p1349 | GCGGCAAGGCAGAAGCAAAGTA | pRL100205 (fixN1) qRT-PCR primer |
| pK19A | ATCAGATCTTGATCCCCTGC | pK19mob mapping primer |
| pK19B | GCACGAGGGAGCTTCCAGGG | pK19mob mapping primer |
| pOTfarforward | GACCTTTTGAATGACCTTTA | PCR mapping primer specific to sequences on Ω cassette |
| pr0074 | TGCCACCGCAATCCTGCTTG | Forward PCR primer for bacA |
| pr0075 | ACGGCATCAAGGGCTTTCGC | Reverse PCR primer for bacA and mapping PCR primer |
| pr0076 | TAGGATCACGAGTTGCAGAT | Mapping PCR primer for bacA |
| pr0077 | GATCGTCGGGCCTGTTGTCG | Forward PCR primer for bacA |
| pr0078 | GAGGACCGCGCCTATGCCGC | Reverse PCR primer for bacA |
| pr0195 | CTCTGATACCGGCGGCATTCTC | RL1651 (ubiG) qRT-PCR primer |
| pr0196 | TGACATCGGTCGCCTTCATCG | RL1651 (ubiG) qRT-PCR primer |