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. 2010 Apr 2;192(11):2892–2899. doi: 10.1128/JB.01535-09

FIG. 4.

FIG. 4.

Evidence for an operon. (A) Northern hybridization with edd (a), glk (b), and eda (c) probes (cf. Fig. 1B). Lane 1, AM511 (wild type); lane 2, HRD1 (edd mutant); lane 3, HRD2 (glk mutant); lane 4, HRD3 (eda mutant); lane 5, HRD4 (ywtG mutant). 23S and 16S indicate the positions of rRNA markers. (B) Demonstration of the ywtG gene transcript. Lane 1, 100-bp DNA ladder; lane 2, PCR product 1 (cf. Fig. 1B) made with primers RT-1 and RT-2 and genomic DNA from the wild type as a template (positive control); lane 3, the same as lane 2 but with total RNA from the wild type as a template (negative control); lane 4, the same as lane 2 but with cDNA for wild-type RNA (synthesized using primer RT-0) as a template; lanes 5, 6, and 7, the same as lanes 2, 3, and 4, respectively, but with the ywtG mutant HRD4 instead of the wild type; lane 8, 1-kb DNA ladder. (C) Transcription of the glk-eda-ywtG region in the wild type. Lanes 1 through 4, the same as the corresponding lanes in panel B, serving as references; lanes 5, 6, and 7, the same as lanes 2, 3, and 4, respectively, but for demonstrating PCR product 2 (cf. Fig. 1B) made with primers RT-1 and RT-3; lane 8, 9, and 10, the same as lanes 2, 3, and 4, respectively, but for demonstrating PCR product 3 (cf. Fig. 1B) made with primers RT-1 and RT-4; lane 11, 1-kb DNA ladder.