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. 2010 Mar 26;192(11):2839–2851. doi: 10.1128/JB.01557-09

FIG. 9.

FIG. 9.

LC-MS analysis of 6-DMAI-3-carbaldehyde and 6-dimethylallyl-l-Trp from culture extracts of the wild-type strain and the ΔiptA mutant strain. The culture, extraction, and LC-MS analysis were performed as described in Materials and Methods. Authentic standards of 6-DMAI-3-carbaldehyde (A) and 6-dimethylallyl-l-Trp (D) were analyzed. The production of 6-DMAI-3-carbaldehyde from the MPLC fraction of the wild type (B) or the ΔiptA mutant strain (C) was analyzed. The production of 6-dimethylallyl-l-Trp from n-butanol extracts of the wild type (E) and the ΔiptA mutant strain (F) was analyzed. The chromatograms shown in panels A, B, and C are the extracted ion chromatograms of m/z 212 [M − H]−. The chromatograms shown in panels D, E, and F are the extracted ion chromatograms of m/z 273 [M + H]+.