FIG. 5.

Effects of proteinase K, detergents, and urea on OMF-associated proteins. (A) SDS protein extract from OMFs was analyzed by SDS-PAGE before or after proteinase K treatment. (B) SDS protein extract from OMFs was analyzed by SDS-PAGE after treatment with 4 M urea, 2% LDAO, or 1% octylglucoside and centrifugation at 65K × g. Proteins solubilized by LDAO or octylglucoside were precipitated from the 65K supernatant before analysis. Molecular masses are given in kDa. Bands numbered from 1 to 6 were subjected to fingerprint analysis.