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. 2010 Apr 2;192(11):2816–2829. doi: 10.1128/JB.00101-10

FIG. 6.

FIG. 6.

MS analysis of intact PilE carrying DATDH- or GATDH-based glycan forms. ESI-MS analyses of intact PilE utilizing pili from strains carrying either pglBMC58 or pglB28013 in different pgl backgrounds were carried out to characterize the glycan structure. N400 pglBMC58 pglA produced two major signals that are enclosed in boxes and represent PilE carrying the DATDH monosaccharide with one (m/z 17530) or two (m/z 17653) PE modifications. The N400 pglB28013 pglA signals represent PilE carrying the GATDH monosaccharide with one (m/z 17576) or two (m/z 17699) PEs. For N400 pglBMC58 and N400 pglB28013, the major signals represent PilE with one or two PE modifications in conjunction with the Ac-Hex-DATDH and Ac-Hex-GATDH disaccharides, respectively (indicated by m/z values in boxes). For N400 pglBMC58 pglEon, major signals correspond to PilE carrying Hex-Hex-DATDH with one or two PE modifications (boxes). N400 pglB28013 pglEon major signals are enclosed in boxes and represent Hex-Hex-GATDH with one or two PEs. Table S1 in the supplemental material shows all of the ion species along with the m/z values and corresponding molecular weights. The strains used were KS306 (N400 pglBMC58 pglA), KS309 (N400 pglB28013 pglA), KS305 (N400 pglBMC58), KS308 (N400 pglB28013), KS307 (N400 pglBMC58 pglEon), and KS310 (N400 pglB28013 pglEon).