FIG. 8.

Protein-associated GATDH monosaccharide is both immunogenic and antigenic. An immunoblot of whole-cell lysates from strains with defined pgl backgrounds was first incubated with pGAb, a polyclonal antiserum raised against pili bearing the GATDH monosaccharide. The same filter was subsequently reprobed with the npg1, npg2, and npg3 MAbs with washing and developing steps between exposures. The strains used all had a 4/3/1 background, in which pilE was conditionally repressed, and were KS105 (4/3/1 pglC), KS122 (4/3/1 pglA), KS101 (4/3/1), KS127 (4/3/1 pglE_on), KS312 (4/3/1 pglB2₈₀₁₃ pglA), KS311 (4/3/1 pglB2₈₀₁₃), and KS313 (4/3/1 pglB2₈₀₁₃ pglE_on).