FIG. 9.
Glycan MAbs react specifically with intact pili as determined by immunoelectron microscopy. Immunogold labeling and transmission electron microscopy using the monoclonal antibodies (npg1, npg2, and npg3) against different N400 pgl strains demonstrated their specificity by binding to pili of the corresponding strains. The strains used were KS100 (N400) (wild type [wt]), KS141 (N400 pglA), KS104 (N400 pglC), KS142 (N400 pglEon), KS144 (N400 pglI), and KS304 (N400 pglEon pglI). Note that for the npg2 and npg3 MAbs, the levels of immunoreactivity were dramatically enhanced in the pglI background, in which glycan O acetylation was not present.