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. 2010 Apr 5;30(11):2799–2810. doi: 10.1128/MCB.00157-10

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FIG. 3. — Terminal differentiation of chondrocytes and formation of the primary ossification center are delayed in Osx-Cre^+/− Capn4^flox/flox embryos. Histology and in situ hybridization analysis of proximal tibias isolated from E14.5 (A) and E15.5 (B) embryos. (A) MMP13 mRNA expression was markedly reduced in Osx-Cre^+/− Capn4^flox/flox embryos. Yellow arrows indicate MMP13 mRNA expression. (B) A spatially restricted expression of Col.10, OP, and MMP13 was observed in Osx-Cre^+/− Capn4^flox/flox in comparison with that in control littermates.