TABLE 1.
Electrical parameters and quantitative index of absorption and secretion in the NOX1KO mouse colona
| Area analyzed and mouse group | Isc (μA/cm2) | Conductance (mS/cm2) | Response to challenge with: |
|
|---|---|---|---|---|
| Butyrate (ΔIsc, μA/cm2) | Carbachol (ΔIsc, μA/cm2) | |||
| Proximal colon | ||||
| Wild-type mice | 41.7 ± 7.3 | 29.4 ± 3.6 | 4.2 ± 1.7 | 37.5 ± 9.5 |
| NOX1KO mice | 26.7 ± 5.9* | 29.5 ± 3.6 | 4.2 ± 1.1 | 9.2 ± 4.5* |
| Distal colon | ||||
| Wild-type mice | 60.0 ± 14.3 | 33.4 ± 3.5 | 5.0 ± 1.8 | 95.0 ± 8.4 |
| NOX1KO mice | 36.0 ± 9.7* | 32.6 ± 4.2 | 5.3 ± 1.8 | 60.0 ± 14.0* |
a
Note significant reductions of baseline short-circuit current (Isc) and carbachol-induced chloride secretion without change in conductance or absorption of butyrate in NOX1KO versus WT mice. Butyrate concentration, 10 mM; carbachol concentration, 100 mM. Data are means ± SEM of 3 separate experiments with sections of both proximal and distal colons from 6 animals per group. *, significantly different from the control value (P < 0.05) by one-way analysis of variance with the Turkey-Kramer multiple comparison post hoc test.