FIG. 6.

ChIP analysis of TIP60 and p400 binding during p21 gene activation. U2OS cells that stably express Flag-tagged p400 (polyclonal cells selected by puromycin) were used for chromatin immunoprecipitation analyses before and after bleomycin treatment. The values obtained with the mock antibody (anti-GAL4 antibody) in real-time PCR analyses were set to 1, and relative values were calculated for each of the other antibodies indicated at the bottom of each panel. The endogenous p21 gene regions monitored were the kb −2.3 p53 response element (top), the kb −1.4 p53 response element (RE) (middle), and a kb +9 control region (bottom).