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. 2010 Apr 5;30(11):2608–2620. doi: 10.1128/MCB.00208-09

FIG. 1.

FIG. 1.

RASSF2 interacts with PAR-4 at physiologically relevant levels. Lysates with equal protein concentrations from H441 lung cancer cells were immunoprecipitated with an anti-RASSF2 antibody (lane 1) or control IgG (lane 2), fractionated on SDS gels, and immunoblotted (IB) with an anti-PAR-4 antibody. Lane 3 is a mock IP of a buffer with no lysate. The endogenous interaction between RASSF2 and PAR-4 was confirmed by the presence of PAR-4 in the proteins precipitated with the RASSF2 antibody (lane 1) and not with control IgG (lane 2). Lane 4 is a PAR-4 positive control lysate.