TABLE 1.
SATB1 interaction partners
| Protein | Interaction strengtha | Minimal interaction domainb | GenBank accession no. |
|---|---|---|---|
| SUMO/Ub conjugation | |||
| SUMO-1 | 627 | 6-101 | U83117 |
| Ubc9 | 814 | 3-158 | U66818 |
| EDD | 330 | 2,481-2,799 | AF015051 |
| TOPORS | 108 | 288-628 | AF098300 |
| Polycomb (Pc2) | 1,636 | 290-558 | AF013956 |
| PIAS family members | |||
| PIAS1 | 2,359 | 9-651 | AF167160 |
| PIAS3 | 1,449 | 185-619 | AB021868 |
| PIASy | 1,186 | 370-510 | AF077952 |
a
Expressed as units of β-galactosidase activity; strongly interacting positive control (pLexA-p53/pB42 AD-T) = 1,000; negative control (pLexA-laminC, pLexA) = 15; at least three replicates each.
b
All y2h proteins interact with SATB1 at sites including their C termini, except for TOPORS (1,045 residues total), which interacts through a 340-residue internal site.