TABLE 2.

Primers for PCR and RT-PCR

Primer function and name	Sequencea
PCR
LetS F	5′-AATAATGCAGTCCTTACCC-3′
LetS R	5′-TGGATGACACCACAAGC-3′
LetS Mut. His F	5′-TTATTGCCAACATGAGTCAAGAAATTCGTACCCCAATGAATGGC-3′
LetS Mut. His R	5′-GCCATTCATTGGGGTACGAATTTCTTGACTCATGTTGGCAATAA-3′
LetS Mutagenesis F	5′-CATGAGTCATGAAATTCGTATGCCAATGAATGGCGTGATTGG-3′
LetS Mutagenesis R	5′-CCAATCACGCCATTCATTGGCATACGAATTTCATGACTCATG-3′
LetS Mut Seq F	5′-CGA TTG CGT CGA AGT ATG-3′
RT-PCR
CsrA_RT_F	5′-TTTGACTCGGCGTATAGGTG-3′
CsrA_RT_R	5′-TTCCTAAGCGAACTTGATTGC-3′
RsmY_RT_F	5′-ATGGATATGTCTGACAGGAAGTC-3′
RsmY_RT_R	5′-ATTAGAGAATAAGTGCTGCATCC-3′
RsmZ_RT_F	5′-TGGATATGAGTCGTGCAAATGG-3′
RsmZ_RT_R	5′-GACTCAGCCCTGGCTTTTC-3′

^aUnderlined nucleotides indicate site changes.