TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| 197N | Parental B. avium strain; Strs Nalr Kms Hag+ | 33 |
| 197N2 | 197N; spontaneous Strr mutant | This study |
| P206b | 197N except hagA::TnphoA206 Kmr Hag− | This studyb |
| P207a | 197N except hagA::TnphoA207 Kmr Hag− | This study |
| G145 | 197N except hagA::TnlacZ145 Kmr Hag− | This studyc |
| P225a | 197N except hagA::TnphoA225 Kmr Hag− | This study |
| P218a | 197N except hagA::TnphoA218 Kmr Hag− | This study |
| P215b | 197N except hagA::TnphoA215 Kmr Hag− | This study |
| P208a | 197N except hagB::TnphoA208 Kmr Hag− | This study |
| P212a | 197N except hagB::TnphoA212 Kmr Hag− | This study |
| P205a | 197N except hagB::TnphoA205 Kmr Hag− | This study |
| P201a | 197N except hagB::TnphoA201 Kmr Hag− | This study |
| PAS666 | 197N2 except ΔhagA Kms Hag− | This study |
| PAS667 | 197N2 except ΔhagB Kms Hag− | This study |
| S17.1 λ pir | E. coli conjugation donor; Strr Nals Kms | 9 |
| HB101 | E. coli cloning strain | Laboratory collection |
| DH5α | E. coli cloning strain | Invitrogen |
| M13/pREP4 | E. coli cloning strain containing plasmid pREP4 | Qiagen |
| Plasmids | ||
| pLAFR5 | Broad-host-range cloning vector | 16 |
| pUC19 | E. coli cloning vector | 36 |
| pCR2.1TOPO | E. coli cloning plasmid; Kmr Apr | Invitrogen |
| pQE30 | His tag cloning vector | Qiagen |
| pQE80-L | His tag cloning vector | Qiagen |
| pKAS46 | oriR6K oriT rpsL Apr Kmr | 30 |
| phagA | pLAFR5 hagA | This study |
| phagB | pLAFR5 hagB | This study |
| pΔhagA | pTOPO ΔhagA | This study |
| pΔhagB | pTOPO ΔhagB | This study |
| pKASΔhagA | pKAS46 ΔhagA Kmr | This study |
| pKASΔhagB | pKAS46 ΔhagB Kmr | This study |
| pΩhagAhis | pQE30 ΩhagAhis | This study |
| pΩhagBhis | pQE80-L ΩhagBhis | This study |
Abbreviations: Hag, phenotypic characteristic of hemagglutination; Strr, streptomycin resistant; Nalr, nalidixic acid resistant; Nals, nalidixic acid sensitive; Kms, kanamycin sensitive; Kmr, kanamycin resistant; Apr, ampicillin resistant. Sequences for each hagA and hagB mutant allele are available at GenBank, with accession numbers assigned as follows: hagA::TnphoA206, FJ001329; hagA::TnphoA207, FJ001327; hagA::TnlacZ145, FJ001328; hagA::TnphoA225, FJ001330; hagA::TnphoA218, FJ001331; hagA::TnphoA215, FJ001332; hagB::TnphoA208, FJ001333; hagB::TnphoA212, FJ001334; hagB::TnphoA205, FJ001335; hagB::TnphoA201, FJ001336; ΔhagA, GQ240879; and ΔhagB, GQ240878.
This strain has been previously reported (33), but the map location of the lesion conferring the Hag− phenotype was not known.
The strain has been previously reported (28), but the map location of the lesion conferring the Hag− phenotype was not known.