FIG. 3.

CT153 is expressed during the mid-developmental cycle and is processed to 57-kDa and 41-kDa polypeptides. C. trachomatis-infected HeLa 229 cells were harvested at the times indicated, and cell lysates were separated by SDS-PAGE, blotted to nitrocellulose, and probed with Abs against CT153, MOMP, or GAPDH. Full-length CT153 was detected during the middle to late development cycle, and immunoreactive polypeptides of 57 kDa and 41 kDa in equimolar ratios indicated processed forms of CT153. U, uninfected.