FIG. 4.

CT153 distributes into insoluble and soluble fractions of homogenized infected cells. Western blots of insoluble (A) and soluble (B) fractions of C. trachomatis-infected L929 cells at various hours postinfection were probed with anti-CT153 Abs. (A) Insoluble fractions contained p91, p57, and p41. The CT153 Ab reacted with an ∼70-kDa polypeptide that may represent an intermediate processed form. (B) Only p91 was detected in soluble fractions. The lower-molecular-weight band in the soluble fractions was nonspecific, as it was also detected in uninfected cells. Monoclonal Abs against MOMP and CPAF were used for positive controls for insoluble and soluble proteins, respectively.