FIG. 1.

Construction of the CvfA⁻ mutant and its cis-complemented strain. The integrational plasmid (pSPC18::′cvfA′) shown at the top was used to disrupt the cvfA locus in S. pyogenes HSC5. The broken ends of the plasmid-encoded box indicate that the cvfA gene segment lacks the 5′ and 3′ ends of the corresponding chromosomal open reading frame, and the region is indicated by the open box above the cvfA gene. The other integrational plasmid (pCIV2::cvfA) shown in the middle was used to complement the disrupted cvfA in the CvfA⁻ mutant. Chromosomal structures resulting from the single recombination event are indicated below the solid vertical arrows. The open reading frames labeled aad9 and aphA3 encode a spectinomycin-resistant determinant and a kanamycin-resistant determinant, respectively. acetyl-CoA, acetyl coenzyme A.