TABLE 3.
Virulence factors significantly upregulated or downregulated in the CvfA− mutant compared to the wild-type control strain
| Locusa | Geneb | Gene productb | Fold change by microarrayc | Fold change by real-time PCRd |
|---|---|---|---|---|
| SPy-1979 | ska | Streptokinase A precursor | 9.28 | 21.8 ± 4.6 |
| SPy-0212 | speG | Exotoxin G precursor | 6.24 | ND |
| SPy-1273 | cfa | CAMP factor | 5.29 | 14.2 ± 4.0 |
| SPy-0167 | slo | Streptolysin O precursor | 3.82 | 4.8 ± 0.8 |
| SPy-0378 | hlyX | Putative hemolysin | 3.76 | ND |
| SPy-2018 | emm | M protein (type 1) | 3.74 | 14.5 ± 7.3 |
| SPy-1054 | Putative collagen-like protein | 3.65 | ND | |
| SPy-0591 | Putative protease | 2.63 | ND | |
| SPy-1896 | ropA | Transcription regulator (trigger factor, prolyl isomerase) | 2.48 | ND |
| SPy-0165 | nga or spn | Nicotine adenine dinucleotide glycohydrolase precursor | 2.43 | ND |
| SPy-2025 | isp | Immunogenic secreted protein precursor | −2.53 | −1.1 ± 3.1 |
| SPy-2043 | mf | Mitogenic factor | −3.17 | −3.6 ± 0.2 |
| SPy-0738 | sagA | Streptolysin S-associated protein | −4.36 | −2.3 ± 0.6 |
| SPy-2039 | speB | Pyrogenic exotoxin B | −130.54 | −76.7 ± 84.3 |
SPy numbers are assigned on the basis of the genome of serotype M1 group A streptococcus (GAS) strain SF370 (12).
Assignment based on annotation of serotype M1 GAS strain SF370 ORFs (http://www.ncbi.nlm.nih.gov/genomes/altik.cgi?gi=178&db=Genome).
Average of a data set consisting of 18 total microarray hybridizations with 2 independently prepared RNA samples. The values here fulfilled the following criteria: the estimated false discovery rate at the 90th percentile in SAM (significance analysis of microarrays) analysis (40) was ≤0.1%, and the fold change value was at least ±2.0.
Fold change determined by real-time RT-PCR. Data show mean ± standard deviation derived from at least two independent experiments. ND, not determined.