FIG. 3.

The transcriptional organization of the two main operons encoding the VcsVUQ2 and VcsRTCNS2 structural components are depicted in panels A and D. Primer pairs were designed to amplify the regions shown by the bars above the genes. The open bars above the genes in panels A and D indicate that RT-PCR did not produce an amplicon, whereas the solid bars indicate that an amplicon was obtained using RT-PCR. The numbers above the bars correspond to the gel lanes in panels B, C, E, and F, showing the results of RT-PCR analyses using RNA extracted from cells grown in LB broth with 0.04% deoxycholate as the template (B and E) and PCR using the same primer pairs with gDNA as the template (C and F). Lanes marked “No RT” in panels B and E show representative PCRs conducted using RNA as the template, indicating that no product was observed, consistent with a lack of gDNA contamination.