. 2010 Mar 29;78(6):2377–2384. doi: 10.1128/IAI.00156-10

TABLE 3.

Mini-Tn5 transposon biofilm-negative mutants of E. coli O157:H7 EDL933^e

Mutant	Locus tag	Gene name	Product or function
Bnp1^a	Z3917^b		Hypothetical protein
Bnp2^a	Z4881^b		Putative aldolase
Bnp3^a	Z5856		Putative aspartate carbamoyltransferase
Bnp4^a	Z2256^b		Unknown protein associated with Rhs element
Bnp5^a	Z5890^b		Partial putative integrase
Bnp6^a	L7020^d	espP	Putative exoprotein precursor
Bnp7^a	Z3635		Putative receptor protein
Bnp8^a	Z3182^b	hisD	l-histidinal:NAD⁺ oxidoreductase
Bnp9^a	Z4625	acrE	Protein affects cell membrane permeability
Bnp10	Z0472	yaiH	Putative enzyme
Bnp11^a	Z1555^b		Hypothetical protein
Bnp12^a	Z2436	ynaJ	Hypothetical protein
Bnp13^a	Z1921^c		Unknown protein encoded by CP933X
Bnp14^a	Z0151	ecpD	Putative fimbrial chaperone protein
Bnp15^a	Z1456^c/Z1457^c		Hypothetical protein encoded by BP-933W/putative DNA binding protein of BP-933W
Bnp16	Z3592	aroC	Chorismate synthase
Bnp17	Z2026	cls	Cardiolipin synthase
Bnp18^a	L7049^d	hlyB	Hemolysin transport protein
Bnp19^a	Z3497 ^b	glpQ	Glycerophosphodiester phosphodiesterase
Bnp20	Z4099	relA	(p)ppGpp synthetase I
Bnp21	Z5049	waaL	Surface polymer ligase
Bnp22^a	Z3199^b/Z3200^b	wbdP/per	Putative glycosyl transferase/perosamine synthetase
Bnp23	Z5050^b	waaD	LPS biosynthesis enzyme
Bnp24^a	Z2163	ydeH	Hypothetical protein
Bnp25	Z1675/Z1676	csgB/csgA	Minor curlin subunit precursor/curlin major subunit, coiled surface structures
Bnp26^a	Z2086^c		Putative regulator
Bnp27^a	Z3660	yfeA	Hypothetical protein
Bnp28^a	Z5214^b		Hypothetical protein
Bnp29	Z5051^b	waaJ	LPS α1,2-glucosyltransferase
Bnp30	Z1670	csgG	Curli production assembly/transport component
Bnp31^a	Z1212^b/Z1213^b		Hypothetical protein
Bnp32^a	Z1213^b		Hypothetical protein
Bnp33	Z2012/Z2013	galU/hns	Glucose-1-phosphate uridylyltransferase/DNA binding protein; pleiotropic regulator
Bnp34	Z3195/Z3196	manC/wbdQ	Mannose-1-phosphate guanosyltransferase/GDP-mannose mannosylhydrolase
Bnp35^a	Z0340^c		Unknown protein encoded in prophage CP933I
Bnp36	Z1676	csgA	Curlin major subunit, coiled surface structures
Bnp37	Z0905	tolQ	Inner membrane protein
Bnp38	Z5054	waaP	LPS biosynthesis enzyme
Bnp39^a	Z4328^b		Hypothetical protein
Bnp40^a	Z0700^b		Putative receptor
Bnp41^a	Z3918^b		Putative chaperone protein
Bnp42^a	Z1494^c		Unknown protein encoded by BP-933W
Bnp43^a	Z0021^b		Hypothetical protein
Bnp44^a	L7047/L7048^d	hlyC/hlyA	Hemolysin protein
Bnp45^a	Z1977	ychM	Hypothetical protein
Bnp46^a	Z0408/Z0409	yahE/yahF	Hypothetical protein/putative oxidoreductase subunit
Bnp47^a	Z3197	fcl	Fucose synthetase
Bnp48^a	Z0904	ybgC	Hypothetical protein
Bnp49^a	Z4327^b		Hypothetical protein
Bnp50	Z5392	dsbA	Protein disulfide isomerase I
Bnp51^a	Z1177^b/Z1178^b		Partial putative phage inhibition protein/putative receptor

Genes not previously shown to be involved with biofilm formation.

On O islands.

Carried in prophage.

On pO157.

A slash indicates that the transposon site was between the genes indicated.