FIG. 4.

In vitro priming of total splenic CD8⁺ T cells or naïve CD8⁺ T cells by DC. Splenic DC from WT mice (4 mice/group) were isolated and incubated overnight with E. cuniculi spores. The next day, purified naïve CD8⁺ T cells or total splenic CD8⁺ T cells were added to the culture. After 72 h of incubation, cells were assessed for IFN-γ, KI67, CD25, and CD69 expression by flow cytometry. The experiment was performed twice, and the data are representative of one experiment.