FIG. 6.

The levels of expression of Yops and YadA are not affected in Y. enterocolitica serotype O:8 acyltransferase mutants. (A) SDS-PAGE analysis (the acrylamide concentration was 4% in the stacking gel and 12% in the separation one) and Coomassie brilliant blue staining of proteins from the supernatants of Ca²⁺-deprived cultures. (B) SDS-PAGE analysis (the acrylamide concentration was 4% in the stacking gel and 12% in the separation one) and Coomassie brilliant blue staining of OM proteins purified from strains grown in LB medium either at 21°C or at 37°C. The white arrow marks YadA protein. MW, molecular weight marker; YeO8, wild type; ΔmsbB, YeO8-ΔmsbBGB2; ΔlpxP, YeO8-ΔlpxPGB; ΔhtrB, YeO8-ΔhtrBGB. The results in both panels are representative of three independent experiments.