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. 2010 Mar 22;78(6):2385–2396. doi: 10.1128/IAI.00510-09

FIG. 5.

FIG. 5.

Cellular localization of AdpC. Proteins were separated by SDS-PAGE and analyzed by Western blotting. AdpC was detected with affinity-purified rabbit anti-rAdpC. (A) E. coli V2921 whole-cell lysate proteins (lanes 1, 2, 3, and 4) and outer membrane proteins (lanes 5 and 6), which were derived from cultures grown in the presence (lanes 1, 3, and 5) or absence (lanes 2, 4, and 6) of IPTG. Proteins were derived from E. coli V2921 (lanes 1 and 2) and E. coli V2846 (lanes 3, 4, 5, and 6). (B) P. intermedia 17 outer membrane proteins (lane 2) and E. coli V2846 cell lysate proteins (lane 3). A molecular mass marker is shown in lane 1.