TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| P. gingivalis | ||
| W83 | Wild type | |
| KDP159 | tprA::[ermF ermAM]; Emr | 54 |
| KDP382 | ΔtapB::ermF; Emr | This study |
| KDP383 | ΔtapA::ermF; Emr | This study |
| KDP384 | Δ[tapA-tapB-tapC]::ermF; Emr | This study |
| KDP385 | ΔporT::[ermF-ermAM]; Emr | This study |
| KDP386 | ΔtapC::ermF; Emr | This study |
| E. coli | ||
| XL1-Blue | General-purpose host strain for cloning | Stratagene |
| BL21(DE3) | Host strain for expression vector pET-32a | Nippongene |
| S. cerevisiae | ||
| AH109 | Host strain for yeast two-hybrid system | Clontech |
| Plasmids | ||
| pBluescript II SK(−) | Apr; cloning vector (pBSSK) | Stratagene |
| pET-32a | Apr; expression vector | Novagen |
| pGEX-6P-1 | Apr; expression vector | GE Healthcare |
| pKD357 | Apr Emr; pBSSK containing ermF ermAM cassette located between upstream and downstream sequences of the porT gene | 43 |
| pKD1008 | Apr; pBSSK containing 0.3-kb tapC upstream and 0.5-kb tapC downstream regions | This study |
| pKD1009 | Apr Emr; ermF cassette was inserted into BamHI site of pKD1008 | This study |
| pKD1010 | Apr; pBSSK containing 0.3-kb tapB upstream and 0.5-kb tapB downstream regions | This study |
| pKD1011 | Apr Emr; ermF cassette was inserted into BamHI site of pKD1010 | This study |
| pKD1012 | Apr; pBSSK contains 0.3-kb tapA upstream and 0.5-kb tapA downstream regions | This study |
| pKD1013 | Apr Emr; ermF cassette was inserted into BamHI site of pKD1012 | This study |
| pKD1014 | Apr; pBSSK contains 0.3-kb tapA upstream and 0.5-kb tapC downstream regions | This study |
| pKD1015 | Apr Emr; ermF cassette was inserted into BamHI site of pKD1014 | This study |
| pKD1016 | Apr; pET-32a containing P. gingivalis tapB gene | This study |
| pKD1017 | Apr; pET-32a containing P. gingivalis tapA gene | This study |
| pKD1018 | Apr; pGEX-6P-1 containing P. gingivalis tprA gene | This study |
| pKD1019 | pGBKT7 containing P. gingivalis tprA gene | This study |
r, resistance; Ap, ampicillin.