TABLE 2.
P. gingivalis genes differentially expressed in the tprA mutant cells placed in a mouse subcutaneous chamber compared to those expressed in the wild-type cellsa
| TIGR no. | Identification | Avg. fold difference |
|---|---|---|
| Downregulated genes | ||
| PG0100 | Hypothetical protein | 0.59 |
| PG0162 | RNA polymerase sigma-70 factor, ECF subfamily | 0.57 |
| PG0591 | ISPg5, transposase Orf2 | 0.63 |
| PG0756 | Hypothetical protein | 0.47 |
| PG1055 | Thiol protease | 0.36 |
| PG1972 | Hemagglutinin protein HagB | 0.63 |
| PG1975 | Hemagglutinin protein HagC | 0.64 |
| PG2100 (tapC) | Immunoreactive 63-kDa PG102 antigen | 0.34 |
| PG2101 (tapB) | Hypothetical protein | 0.25 |
| PG2102 (tapA) | Immunoreactive 61-kDa PG91 antigen | 0.51 |
| PG2103 | Hypothetical protein | 0.24 |
| PG2214 | Hypothetical protein | 0.57 |
| Upregulated genes | ||
| PG0327 | Hypothetical protein | 1.92 |
| PG0373 | Hypothetical protein | 1.72 |
| PG0546 | Hypothetical protein | 1.83 |
| PG0635 | Ribosomal protein L11 methyltransferase | 1.69 |
| PG0656 | Ribosomal protein L34 | 2.02 |
| PG0722 | Hypothetical protein | 2.93 |
| PG0969 | S-Adenosylmethionine:tRNA ribosyltransferase-isomerase (putative protein) | 1.98 |
| PG1532 | Hypothetical protein | 1.92 |
| PG1866 | Hypothetical protein | 1.93 |
| PG2006 | Hypothetical protein | 1.69 |
| PG2225 | Hypothetical protein | 1.63 |
a
Mouse subcutaneous chamber experiments were performed as described in Materials and Methods. Total RNA was extracted from strains W83 (wild type) and KDP159 (tprA::erm). The extracted total RNA was analyzed by using a microarray as described in Materials and Methods. Numbers in the “Avg fold difference” column indicate the levels of gene expression in the tprA mutant versus the wild-type strain. The cutoff ratio for the levels of difference was 1.5.