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. 2010 Apr 5;78(6):2438–2445. doi: 10.1128/IAI.00812-09

FIG. 1.

FIG. 1.

Inhibition of IFN-γ-mediated NO production by L. mexicana promastigotes (Pro) and amastigotes (Ama). (A) NO assay of B10R Mφs left untreated (−) (bar 1), stimulated with IFN-γ (100 U/ml) for 24 h (+) (bar 2), infected with Leishmania (overnight [O/N], 20:1 ratio) (+) (bars 3 and 5), or infected with Leishmania (O/N, 20:1 ratio) and then stimulated with IFN-γ (100 U/ml) for 24 h (bars 4 and 6). The values are means plus standard errors of the means (SEMs) (error bars). Values that were significantly different (P < 0.05, analysis of variance [ANOVA] test) are indicated by an asterisk. (B) Western blot analysis of L. mexicana promastigote and amastigote cell lysates (40 μg). The membrane was cut and blotted for gp63 (top panel) and A2 (middle panel). The membrane was then stripped and reblotted for LACK D (bottom panel) to demonstrate equal loading. All results are representative of at least three independent experiments. w.b., Western blotted.