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. 2010 Apr 5;78(6):2438–2445. doi: 10.1128/IAI.00812-09

FIG. 2.

FIG. 2.

Activation of host PTPs by L. mexicana promastigotes and amastigotes. (A) B10R Mφs were infected with L. mexicana promastigotes or amastigotes for the specified time course (0 to 24 h, 20:1 ratio), and total PTP activity of Mφ lysates (20 μg) was measured using the pNPP phosphatase assay. (B) Cell lysates (30 μg) of Mφs infected with promastigotes or amastigotes of L. mexicana for the specified time course (0 to 24 h, 20:1 ratio) subjected to an in-gel PTP assay. The rightmost two lanes contain lysates (30 μg) from WT and SHP-1−/− Mφs to confirm the band corresponding to SHP-1. All results are representative of at least three independent experiments.