FIG. 7.

Nuclear translocation of TFs in response to IFN-γ in Mφs infected with WT or lmcpb^−/− L. mexicana promastigotes (P) and amastigotes (A). B10R Mφs were left uninfected, stimulated with IFN-γ (100 U/ml) for 6 h (+), and infected (overnight [O/N]) with WT or lmcpb^−/− promastigotes and amastigotes of L. mexicana in a 5:1 parasite-to-cell ratio, or they were infected (O/N) and then stimulated with IFN-γ (100 U/ml) for 6 h. (A to C) Nuclear proteins were extracted and subjected to EMSA to evaluate the DNA-binding activity of NF-κB (A), STAT-1α (B), and AP-1 (C). S, specific competition (100-fold excess of specific nonradioactive oligonucleotide); NS, nonspecific competition (100-fold excess of nonspecific, nonradioactive Sp-1 oligonucleotide).