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. 2010 Apr 5;78(6):2857–2867. doi: 10.1128/IAI.01138-09

FIG. 5.

FIG. 5.

The effect of LY294002 on the phagocytosis of L. monocytogenes (LM) and difference in the phosphorylation of Akt between WT, TLR2−/−, and MyD88−/− macrophages after L. monocytogenes infection. (A) WT macrophages were infected with L. monocytogenes at an MOI of 20 in the presence of graded concentrations of LY294002, a PI3K inhibitor, and the number of phagocytosed bacteria was determined. Data are expressed as the means ± standard deviations (SD) for results of triplicate cultures. (B) WT, TLR2−/−, and MyD88−/− macrophages were infected with L. monocytogenes at an MOI of 20 for 1 h in the presence or absence of 20 μM LY294002, and the number of phagocytosed bacteria was determined. Data are expressed as the means ± SD for results of triplicate cultures. Similar results were obtained in two independent experiments. *, P < 0.05. (C) WT, TLR2−/−, and MyD88−/− macrophages were infected with L. monocytogenes at an MOI of 20 for 15 and 30 min. Cells were lysed, and phosphorylated Akt was evaluated by Western blotting. Total Akt was used as a loading control.