FIG. 3.

Kinetics of responding memory subpopulations of p18-specific CD8⁺ T cells and the surface expression of CD27 on these lymphocyte subpopulations. Mice were primed by i.m. inoculation with 20 μg DNA expressing HIV-1 HXB2 gp120 protein or the pulmonary inoculation of this DNA formulated with PEI. (A) p18-specific CD8⁺ T cells were divided into effector (CD62L^lo CD127^lo) (E), effector memory (CD62L^lo CD127^hi) (EM), and central memory (CD62L^hi CD127^hi) (CM) subpopulations. (B) Expression of CD27 on effector, effector memory, and central memory p18-specific CD8⁺ T cells. Data represent means from five to seven mice per group ± SE.