FIG. 4.

Effect of the route of immunization on pulmonary p18-specific CD8⁺ T-cell responses. Pulmonary (A) and mediastinal lymph node (MLN) (B) p18-specific CD8⁺ T-cell responses of mice immunized with DNA-gp120 by the i.m. route or DNA-gp120-PEI by the pulmonary route. Pulmonary data were generated from nine mice, and data are represented as the percent tetramer-binding CD8⁺ T cells ± SE. MLN data were generated from three independent pools of three mice. Statistically significant differences between groups were analyzed with Student's t test (***, P < 0.001).