FIG. 8.

Kinetics of secondary memory subpopulations of p18-specific CD8⁺ T cells. Mice primed by the i.m. inoculation of DNA-gp120 or pulmonary inoculation of DNA-gp120-PEI were boosted by the i.m. or pulmonary delivery of the same immunogens. p18-specific CD8⁺ T cells were divided into effector (CD62L^lo CD127^lo), effector memory (CD62L^lo CD127^hi), and central memory (CD62L^hi CD127^hi) subpopulations. (A) Representative flow plots display the different p18-specific CD8⁺ T-cell memory subpopulations in the peripheral blood of mice following the boost immunization. (B) Kinetics of the p18-specific CD8⁺ T-cell memory subpopulations in the peripheral blood of immunized mice. Data represent means from five to eight mice per group ± SE. Differences between groups were analyzed with a one-way ANOVA test (*, P < 0.05; **, P < 0.01).