FIG. 1.

Phenotyping of parent, mutant, and rescue viruses by fluorescence microscopy. MDBK cells were infected with various viruses to be analyzed at 72 hpi. The top two rows show plaques of BAC-derived rBoHV-5 and its mutants, whereas the bottom two rows show BAC-derived rBoHV-1 and its mutants. The top row shows green fluorescent plaques of rBoHV-5 (A), rBoHV-5gD1HA (B), and rrBoHV-5gD5V5 (C). In the second row, the same dishes were stained with mcAbs and a red fluorescent Cy3 conjugate (mcAb against the HA tag [A′ and B′] and mcAb against the V5 tag [C′]). In the third row, the following fluorescent plaques are shown: green fluorescent rBoHV-1 (D), rBoHV-1gD5V5 (E), and yellow fluorescent rrBoHV-1gD1EYFP (F). In the fourth row (D′ and E′), the same viruses as those in the third row were stained with mcAb against V5 and a red fluorescent Cy3 conjugate.