TABLE 1.

Synthetic oligonucleotides used in this study

Oligonucleotide	Sequencea	Amplification product
P1	5′-GATCGAGCTCGTTAACCGACTCGGATTTTGGGC-3′	5′ of gD5 homology arm
P2	5′-GATCGCGGCCGCAGTTGCTCGCTCGCAGCAAC-3′
P3	5′-GATCACCGGTGCGGCCCGGGCCCTCCCCCG-3′	3′ of gD5 homology arm
P4	5′-GATCGGTACCGTTAACGCGGTCGCCTGTAGCATGACGAAGC-3′
P5	5′-ATAAGAATGCGGCCGCATGAAGGGCCGACATTGGCCGTGc-3′	gD1 ORF fused to HA
P6	5′-GATCGAATTCTCAAGCATAATCTGGAACATCATATGGATACCCGGGCAGCGCGCTGTAGt-3′b
P7	5′-GATCGGATCCATGCGGAGGCTGGCGCTGCT-3′	gD5 ORF fused to V5
P8	5′-GATCAAGCTTTCACGTAGAATCGAGACCGAGGAGAGGGTTAGGGATAGGCTTACCCCCGGGCAGCGCGCTGTAGT-3′c
P9	5′-GATCGAGCTCGTTAACCTCCGACTACGCGCTCTACG-3′	5′ of gD1 homology arm
P10	5′-GATCGGATCCGTTCGCCCGCTCGCAGCA-3′
P11	5′-GATCAAGCTTGGGGCCTAGGCCCTCCCCC-3′	3′ of gD1 homology arm
P12	5′-GATCGGTACCGTTAACGCGCCGAGAGCACGGC-3′
P13	5′-GATCTCTAGAACCCGCATCCGCGGTGGCTTT-3′	5′ of gD1 flank and gD1 ORF without stop codon
P14	5′-GATCGGATCCCCCGGGCAGCGCGCTGTAGTT-3′
P15	5′-AGTCGGATCCTGAGCGGCCTAGGCCCTCCCCCGA-3′	3′ of gD1 flank
P16	5′-GCATGAATTCAGGATCGACGCCAGTTGGCGCCGGAA-3′
P17	5′-ACGTGGATCCCATGGTGAGCAAGGGCGAGGA-3′	YFP ORF
P18	5′-GATCGGATCCCTTGTACAGCTCGTCCATGC-3′
P19	5′-GGGCGACTAGAGATACACTCGCCCCGCGCGGCTGCTGCGAGCGGGCGAACcctcgaggtcgacataactt-3′	Kanamycin ORF flanked by 50-bp homology arms for gD1 locus
P20	5′-GGAGCCGGGGCTAGGAGCAAAGGGGGCGGTCGGGGGAGGGCCTAGGCCGCgagcccttaattaaccggtg-3′
gD5upper	5′-CGGAGGCTGGCGCTGCTGT-3′	gD5-DIG-labeled probe
gD5lower	5′-ACAGCGTGCGCCCCACCTGC-3′
gD1upper	5′-GACGACGAGCTGGGACTGATT-3′	gD1-DIG-labeled probe
gD1lower	5′-CGGGGGTCTGACTCTC-3′

^aUnderlining indicates the restriction site used for cloning. The binding part of the primers is shown in lowercase type.

^bThe letters in boldface type indicate an HA tag.

^cThe letters in boldface type indicate a V5 tag.