TABLE 1.

Potential host factors in HCV replication enriched in CRCs^d

Identified protein	Gene name	Protein accession no.	Identification methoda	Differential spot intensityb
Annexin A2	ANXA2	AAH23990	PMF (12, P < 0.0001)	7
			PMF (11, P < 0,0001)
			MSMS (4, P < 0.0001)
			MSMS (10, P < 0.0001)
Annexin A4	ANXA4	AAH63672	PMF (10, P < 0.0001)	2
Annexin A5	ANXA5	AAH01429	PMF (7, P < 0.01)	2
			MSMS (9, P < 0.0001)
ATP synthase, beta subunit	ATP5B	AAH16512	PMF (8, P < 0.01)	4c
			PMF (17, P < 0.0001)
			PMF (7, P < 0.01)
			MSMS (4, P < 0.0001)
ATP synthase, O subunit	ATP5O	AAV38639	PMF (8, P < 0.001)	1
Calnexin	CANX	AAH42843	MSMS (1, P < 0.05)	1
BiPe	HSPA5	CAA61201	PMF (13, P < 0.0001)	1
Peroxiredoxin 3	PRDX3	NP_054817	PMF (5, P < 0.01)	1

^aPMF, peptide mass fingerprint using MALDI-TOF MS data; MSMS, peptide sequencing using HPLC ESI MSMS data. The numbers in parentheses refer to the number of matching peptides and the P value for identity or extensive homology.

^bNumber of experiments in which enrichment of the protein in CRCs of replicon cells was found, based on the comparison of the once identified spots on behalf of their location in the 2D gel; eight repetitions were performed.

^cVarious molecular sizes of 27 and 56 kDa; all spots were identified by PMF or MSMS analysis.

^dProtein spots identified with scores indicating identity (P < 0.05) were included.

^eBiP, binding immunoglobulin protein.