FIG. 6.

UV-MVC inoculation induces cell cycle arrest at G₂/M but not cell death. WRD cells were infected by MVC or inoculated with UV-MVC at an MOI of 9. (A) Infected or inoculated cells were costained with Live/Dead Violet and poly-FLICA peptide at indicated times p.i. Stained cells were plotted in histograms as Live/Dead Violet and FLICA signals. The numbers in the square show percentages of live cells (double negative). (B) At 48 h p.i., mock- or UV-MVC-infected cells were stained with DAPI and plotted as cell counts and DAPI signal. The percentage of each cell cycle phase was quantified and is shown as a pie graph at the bottom of the panel. (C) UV-MVC-infected cells were harvested at indicated times p.i. Cell lysates were subjected to Western blotting analysis using anti-cyclin B1, anti-cdc2 (pY15), anti-cyclin A, and anti-β-actin, respectively. The level of signals on blots is normalized to the level of β-actin and is shown in the bar chart. The normalized value of the mock cells is arbitrarily set to 1. A representative of two independent experiments is shown in panels A and B.