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. 2010 Mar 24;84(11):5508–5519. doi: 10.1128/JVI.02483-09

FIG. 1.

FIG. 1.

Four HLA-A*1101-restricted HIV-1-specific CD8+ T cells in chronically HIV-1-infected HLA-A*1101+ individuals. (A) After PBMC from an HLA-A*1101+ HIV-1-infected and HIV-1-uninfected individuals had been stimulated singly with each of the indicated peptides for 2 weeks, HIV-1-specific CD8+ T cells were detected by measuring IFN-γ-producing CD8+ T cells in the culture after stimulation with the corresponding peptide-pulsed cells. Either Nef84 or Nef84-2L peptide was used for individuals infected with HIV carrying the corresponding sequence. A representative result is shown. (B) Summary of ICC assays for HLA-A*1101+ HIV-1-infected individuals and HIV-1-uninfected individuals. For detection of Nef84- and Nef84-2L-specific CD8+ T cells, Nef84 and Nef84-2L peptides were incubated with cells from individuals infected with the wild type or the 2F and 2L viruses, respectively. The circle symbols and the triangle symbols represent the frequency of IFN-γ-producing CD8+ T cells after stimulation with Nef84 and Nef84-2L peptides, respectively. The average + 3 SD of IFN-γ-producing CD8+ T cells in HIV-1-uninfected individuals was defined as a positive value (Gag349, >0.34%; Pol675, >0.56%; Nef73, >0.32%; Nef84 or Nef84-2L, >0.63%). Dotted lines indicate the average + 3 SD, and solid lines indicate the average in HIV-1-infected individuals.