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. 2010 Mar 24;84(11):5508–5519. doi: 10.1128/JVI.02483-09

FIG. 5.

FIG. 5.

Ability of Nef84-specific CTLs to suppress replication of HIV-1-Nef84-9R mutant virus. (A) Cytolytic activities of Nef84-specific CTL clones in killing C1R-A*1101 cells pulsed with Nef84-9R peptide. C1R-A*1101 cells were prepulsed with various concentrations of Nef84, Nef84-2L, or Nef84-2L9R peptide. Cytolytic activities of Nef84-specific CTL clones were measured at an effector-to-target ratio of 2:1. (B) Ability of Nef84-2L9R peptide to bind HLA-A*1101. The affinity was measured by a stabilization assay using RMA-S-A*1101 cells. (C) Surface expression of HLA class I molecules on CD4+ T cells infected with NL-432-Nef84-2L9R. (D) Ability of each Nef84-specific CTL clone to suppress NL-432-Nef84-2L9R replication in CD4+ T cells. (E) Analysis of ability of all 3 Nef84-specific CTL clones to suppress replication of NL-432 or NL-432-Nef84-2L9R.