FIG. 2.

The coiled-coil domain can condition TRIM5α antiretroviral specificity. MDTF cells expressing the indicated squirrel monkey and human TRIM5α Y³³⁶K chimeras (see Fig. 1A), or transduced with an empty vector (vector), were challenged with serial 2-fold dilutions of MLV-, SIVmac, HIV2-, or HIV1-based GFP vectors initially titered on parental MDTF cells. Infections were scored 72 h postinfection by flow cytometry. The data are representative of at least three independent experiments, and the restriction activities of each of the TRIM5α variants were obtained using two independent stable cell lines.