FIG. 6.

The squirrel monkey coiled-coil domain can efficiently mediate multimerization. Squirrel monkey Pindak cells stably expressing the indicated full-length or truncated versions of TRIM5α were analyzed by Western blotting (upper left panel) and then challenged with HIV1, N-MLV, and SIVmac GFP vectors and analyzed 72 h later by flow cytometry. The figure shows infections performed in duplicate using a single dose of each GFP vector, but similar results were obtained using two other vector doses.