FIG. 3.

JEV-activated NF-κB is composed of p50 and p65. EMSA and supershift assays were performed with nuclear extracts from wild-type and IKK1^−/− MEFs as labeled on top. Lanes 1 and 6, oligomer alone; lanes 2 and 7, uninfected cells; lanes 3 and 8, infected cells obtained at 14 h p.i.; lanes 4 and 9, infected cells obtained at 14 h p.i. along with anti-RelA specific antibodies; lanes 5 and 10, infected cells obtained at 14 h p.i. along with anti-p50 specific antibodies. The arrow indicates the NF-κB-DNA binary complex that is supershifted upon addition of antibodies.